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食品中罂粟碱、那可丁、吗啡、可待因和蒂巴因的测定——高效液相色谱法
陈芩
0
(黄冈大别山检测认证有限公司)
摘要:
S本法建立了高效液相色谱法测定食品中罂粟碱、那可丁、吗啡、可待因和蒂巴因5种非法添加物的检测方法,色谱柱ZORBAX SB-C18(4.6×150 mm 5-Micron),流动相为0.1%甲酸的10 mmol/L乙酸铵溶液-乙腈(10:90),流速0.3 mL/min,检测波长280 nm,进样量10 μL,柱温30 ℃,进行定量分析。在浓度为0.01-10.00μg/mL范围内,标准曲线线性关系良好(相关系数R>0.9990), 罂粟碱、那可丁、吗啡、可待因和蒂巴因的检出限分别是:0.05 μg/mL、0.03 μg/mL、0.1μg/mL、0.1μg/mL、0.05 μg/mL。五种生物碱加标水平在1.2、1.5 mg/kg时,回收率为88-93%,相对偏差为1.4-3.2%。该方法方便快捷,成本较低,操作简便,分析快速,可用于食品中5种非法添加物检测。
关键词:  S罂粟碱  那可丁  吗啡  可待因  蒂巴因  高效液相色谱法
DOI:
投稿时间:2019-03-12修订日期:2019-03-12
基金项目:
Determination of Papaverine hydrochloride,Noscapine hydrochloride,Morphine,Codeine,Thebaine in foods by HPLC-DAD
()
Abstract:
The method for Papaverine hydrochloride,Noscapine hydrochloride,Morphine, Codeine,Thebaine in foods by HPLC was established. The chromatogram conditions includes ZORBAX SB-C18(4.6×150 mm 5-Micron), 10 mmol/L ammonium acetate solution of 0.1% formic acid and acetonitrile(volume ratio 10:90) as the mobile phase,isocratic elution(speed:0.3 mL/min), ultraviolet Sdetector (UVD,280 nm), 10 μL injection volume and 30 ℃ column temperature. n the concentration range of 0.01-10.00 μg / mL, the linear relationship between the standard curve was good (correlation coefficient R> 0.9990), Papaverine hydrochloride, The detection limits of Noscapine hydrochloride,Morphine,Thebaine are 0.05 μg/mL、0.03 μg/mL、0.1μg/mL、0.1μg/mL、0.05 μg/mL. The recoveries of five alkaloids were 88-93% and the relative deviations were 1.4-3.2% at the standard addition levels of 1.2 and 1.5 mg/kg. The method is convenient, quick, low in cost, simple in operation, fast in analysis, and can be used for detecting five illegal additives in food.
Key words:  Papaverine hydrochloride  Noscapine hydrochloride  Morphine  Thebaine  Codeine  HPLC

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