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食用鱼类产品中β-溶血性嗜水气单胞菌毒性基因的多重PCR检测技术研究
余文杰
0
(漳州职业技术学院)
摘要:
本研究目的是建立检测食用鱼类产品中β-溶血性嗜水气单胞菌气溶素基因(aerA)和溶血素基因(ahh1)的技术。气溶素和溶血素是β-溶血性嗜水气单胞菌重要的毒力因子。多重PCR技术被应用于检测28个来源于食用鱼类产品的β-溶血性嗜水气单胞菌样本,包括鲤鱼片,水,鲫鱼片、鳟鱼片、鲢鱼片、鲑鱼片和带鱼片。该研究使用标准菌株嗜水气单胞菌ATCC7965和分离于死蟒蛇的带有β-溶血性毒素基因的嗜水气单胞菌作为对照。所有菌株样本(100%)被证实携带嗜水气单胞菌16srRNA基因(356个碱基对)。所有嗜水气单胞菌样本(100%)被证实携带ahh1毒性基因 (130个碱基对)。26个嗜水气单胞菌样本(93%)被证实携带aerA基因(309个碱基对)。本研究证实β-溶血性嗜水气单胞菌菌株广泛存在于供人类食用的鱼类产品中,对消费者产生了一定的健康风险。
关键词:  嗜水气单胞菌,多重PCR、气溶素,溶血素
DOI:
投稿时间:2019-02-22修订日期:2019-02-22
基金项目:福建省科技厅科学研究项目(JK2012012)
Multiplex PCR Detection of Haemolysin Genes in β-Haemolytic Aeromonas Hydrophila Strains Isiolated from Fish Product
()
Abstract:
The aim of this study was to establish the presence of genes coding aerolysin (aerA) and haemolysin (ahh1) in β-haemolytic Aeromonas hydrophila strains isolated from fish and fish products available for human consumption throughout food stores. Aerolysin and haemolysin are viewed as important virulence factors related to the entero-toxigenicity of A. hydrophila. A multiplex PCR was applied to 28 β-haemolytic A. hydrophila strains isolated from carp cutlets, water, crucian cutlets, trout cutlets, silver carp cutlets, trout cutlets and hailtail cutlets. The study used a reference A. hydrophila strain (ATCC 7965) and an A. hydrophila strain isolated from a dead anaconda, as positive controls with strong β-haemolytic activity. All strains (100%) possessed the chromosome gene 16S rRNA (356 bp) specific for A. hydrophila. In all (100%) A. hydrophila strains the gene ahh1 was present (130 bp). The gene aerA was confirmed (309 bp) in 26 A. hydrophila strains (93%). The presence of β-haemolytic A. hydrophila strains in fish and fish products available for human consumption throughout food stores represent a risk to the consumer’s health.
Key words:  A. hydrophila, PCR, aerolysin, haemolysin, β-haemolysis

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