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以Lorke法为基础的半致死剂量(LD50)分析方法在油酸、化香树果序、黄连素抗镉减毒评价中的应用
房志家,孙力军
0
(广东海洋大学食品科技学院)
摘要:
镉污染严重着威胁农业的健康发展,如何有效抗镉减毒是保障农业生产安全的重要措施,然而目前缺乏高效的抗镉药物及简便易行的评价方法。本实验借助Lorke法为基础的半致死剂量(LD50)分析方法,检测了油酸、化香树果序、黄连素等不同潜在的抗镉化合物的减毒作用。通过改良后的Lorke法测定不同抗镉药物对小鼠的径口镉LD50的影响。结果显示,与200mg/kg维生素C相比,在灌胃200mg/kg油酸、化香树果序、黄连素能够减轻镉的毒性,提高镉的LD50。对照的LD50由140-160mg/kg分别上升至300-320mg/kg(油酸组),280-300mg/kg(化香树果序组)和320-340mg/kg(黄连素组)。以上结果表明,以Lorke法为基础的LD50分析方法,能够评价出油酸、化香树果序、黄连素的减镉毒作用,及其剂量-效应关系。以上结果同时证明了相同剂量下黄连素效果要优于油酸、优于化香树果序。
关键词:  镉毒性  油酸  黄连素  化香树果序  半致死剂量
DOI:
投稿时间:2019-01-08修订日期:2019-01-08
基金项目:国家自然科学基金(31701706); 广东省高等学校创新强校项目(GDOU2019040203)
Application of Lorke’s method-based median lethal dose LD50 analysisin evaluation of oleic acid, platycarya strobilacea caryopsis, berberine on resistance and attenuation of cadmium toxicity
,SUN Li-jun
(College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Guangdong Provincial Engineering Technology Research Center of Marine Food,Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution)
Abstract:
Cadmium pollution is a serious threat to the development of agriculture, control and reduction of cadmium is an important way to ensure the agricultural production and safety. However, sufficient methods for controlling and evaluating cadmium toxicity are not available currently. To effectively reduce the toxic and adverse effects caused by cadmium, the Lorke’s method-based LD50 analysis was used to evaluate the attenuating effect of oleic acid, platycarya strobilacea caryopsis, berberine on cadmium toxicity. The acute oral toxicity of chloride cadmium in mice was tested and LD50 was determined using Lorke’s method-based two-step process. The results showed that 200mg/kg of oleic acid, p. strobilacea caryopsis and berberine could reduce the toxicity of cadmium and increase the LD50 of cadmium in mice compared with 200mg/kg of vitamin C. Their LD50 of cadmium rose to 300-320mg/kg, 280-300mg/kg and 320-340mg/kg respectively. The results indicated that the LD50 analysis based on Lorke’s method is feasible to evaluate the attenuating effect of oleic acid, p. strobilacea caryopsis and berberine on cadmium toxicity, as well as their dose-effect relationship. The results also proved that the attenuating effect of berberine on cadmium toxicity was better than oleic acid and p. strobilacea at the same dose.
Key words:  cadmium toxicity  oleic acid  berberine  platycarya strobilacea caryopsis  medial lethal dose

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